Argumentative Research Paper Parents Responsible for...

â€Å"Parental behavioral patterns concerning shopping, cooking, eating and exercise have an important influence on a child’s energy, balance and ultimately their weight† said diet specialist, Anne Collins (â€Å"Childhood Obesity Facts†). Childhood obesity has more than tripled since the 1980’s (â€Å"Childhood Obesity Facts†). Childhood obesity often leads to obesity as an adult which can put a person at greater risks dealing with the heart, diabetes, and many other obese related diseases. People want to blame the schools and today’s technology for childhood obesity, in reality, the responsibility lies in the hands of their parents. Parents are at fault for childhood obesity. The job of a parent is to teach a child wrong from right, including their†¦show more content†¦Parents need to realize that they are putting their own children’s lives at jeopardy by making them more likely to develop diseases later in life. In todayâ €™s society, technology has played a big role in everyone’s lives and has taken over. Kids today stay inside and play on the computer, watch TV, play video games, or mess with tablets and smartphones. Several people find other, more entertaining things to do than play outside and be active. Some of the times, physical education in schools is the only physical activity students have on a daily basis (â€Å"Obesity†). Kids and teenagers would much rather be wrapped up in a social network than going for a job or going to the gym. Back in the day, playing outside was one of the few entertainments. Parents have no discipline and let their children do what they want to their bodies. Technology has caused people to be less active and not as self-controlled causing them to be obese or overweight later in life. â€Å"Children and adolescents who are obese are more likely to be obese as adults, putting them more at risk for heart disease, type 2 diabetes, stroke, types of cancers, osteoarthritis, bone and joint problems, sleep apnea, increases risk for many types of cancer including breast, colon, endometrium, esophagus, kidney, pancreas, gall bladder, thyroid, ovary, cervix, and prostate† (â€Å"Childhood Obesity Facts†). A person can increase the risk of getting several diseases if having bad eating habits, exerciseShow MoreRelatedDefinition of Adolescent Development14194 Words   |  57 Pagesinclude predictable physical and mental milestones. Introduction Derived from the Latin verb adolescere (to grow into maturity), adolescence is the period of transition from childhood to adulthood. Adolescent is a distinct and dynamic phase of development in the life of an individual. It is a period of transition from childhood to adulthood and is characterized by spurts of physical, mental, emotional and social development. WHO considers adolescence to be the period between 10-19 years of age, whichRead MoreDefinition of Adolescent Development14200 Words   |  57 Pagesinclude predictable physical and mental milestones. Introduction Derived from the Latin verb adolescere (to grow into maturity), adolescence is the period of transition from childhood to adulthood. Adolescent is a distinct and dynamic phase of development in the life of an individual. It is a period of transition from childhood to adulthood and is characterized by spurts of physical, mental, emotional and social development. WHO considers adolescence to be the period between 10-19 years of age, whichRead MoreFor Against by L.G. Alexander31987 Words   |  128 Pagestraffic laws can prevent accidents 10 Parents are too permissive with their children nowadays II 12 Advertisers perform a useful service to the community Pop stars certainly earn their money 13 Vicious and dangerous sports should be banned by law 14 Transistor radios should be prohibited in public places 15 The only thing people are interested in today is earning more money 16 Compulsory military service should be abolished in all countries 17 Childhood is certainly not the happiest timeRead MoreStephen P. Robbins Timothy A. Judge (2011) Organizational Behaviour 15th Edition New Jersey: Prentice Hall393164 Words   |  1573 PagesFoundations of Organization Structure 479 v vi BRIEF CONTENTS 4 The Organization System 16 Organizational Culture 511 17 Human Resource Policies and Practices 543 18 Organizational Change and Stress Management 577 Appendix A Research in Organizational Behavior Comprehensive Cases Indexes Glindex 637 663 616 623 Contents Preface xxii 1 1 Introduction What Is Organizational Behavior? 3 The Importance of Interpersonal Skills 4 What Managers Do 5 Management

Essay on Theories Related to Teen Pregnancy - 2530 Words

The concept of comprehensive social intervention has been defined as the process of identifying social problems in an attempt to eradicate them. In looking at the broad range of social characteristics and the behavior associated with teen pregnancy, it is obvious that the emphases placed on the effort to recognize and alleviate teen pregnancy can be celebrated through the effectiveness of education, family planning, and abstinence. However, the attempt to analyze and deal with the cause-and-effect relationship with teen pregnancy is an attempt in understanding the social world itself. In 2006, statistics show that there was a significant increase in teen pregnancy after a decade long decrease. The potential for understanding this increase†¦show more content†¦This paper provides resources and references to show how each perspective can apply to teen pregnancy and the increase in rates seen in 2006. Literature Review Looking at the theories Symbolic Interactionism and Structure-Functional Gender is a characteristic that distinguishes between male and female. Gender roles are often defined through both the social and cultural realms. Beliefs, attitudes, and characteristics all come together to shape an individual. Men and women give meaning to parenthood based on discourses portrayed within their culture. According to the structural-functional perspective in sociology, the family is a social institution that performs certain essential functions for society (Lamanna Reidman, 2006). This theoretical perspective draws attention to functions performed by the family, as a whole, as well as the functionality of specialized gender roles within the family unit. Consequently, a number of social problems faced by many Americans today can be connected to the absence of a father both emotionally and physically. Exploring the social context of gender roles in parenting highlights the influential patterns that allow researchers to draw conclusions about people’s expectati ons when it comes toShow MoreRelatedHealth Belief Model: Condoms and Sexual Risk Essay1458 Words   |  6 PagesSexual Risk Teen Pregnancy Prevention (Condom) Section 1- Health Belief Model The health belief model (HBM) is a psychosocial model that was developed during the 1950’s by social psychologists of the U.S public health services, and this model was used to explain why people failed to participate in disease prevention and detection programs. The theory is one of the most health behavior theories, which is used to explain behavior change and maintenances, but often times used to predict behaviorRead MoreEssay on Factors that Lead to Teen Pregnancy1363 Words   |  6 PagesFactors that Lead to Teen Pregnancy Although the overall rate of teen pregnancy has been declining, the rates have remained high for teens that are most vulnerable. The great majority of Americans believe that teen pregnancies are a serious national problem, indeed a problem that is the major component of what is thought to be national moral decline. However, what causes these teens to become pregnant at such a young age? A large body of research has identified a number of factoresRead MoreQuestions On Gender Identity And Gender Essay1251 Words   |  6 PagesPsychoanalytic Theory of Gender (p.371): This theory stems from Sigmund Freud. It states that from the ages of 3-5 a child has a sexual attraction to their opposite sex parent. Freud claims that as the child grows, they become anxious and ashamed of these feelings and subsequently adopt the mannerisms and characteristics of their same sex parent. I think that this is interesting because Freud was very adamant that even as a young child we had innate sexual desires. 3. Gender Schema Theory (p. 374): ThisRead MoreTeen Pregnancy Norms Affected By Race, Religion, And Social Status1669 Words   |  7 Pages Teen Pregnancy Norms Affected by Race, Religion, and Social Status INTRODUCTION Teen pregnancy is a controversial issue that Americans are facing in society today. The problematic behavior that comes along with teen pregnancy is a cultural phenomenon that exists on many domains. A bulk of the teenage population is starting to believe that teen pregnancy is customary and okay. A persons’ background has a lot to say about how weakly or strongly this norm is enforcedRead MoreTeen Pregnancy: A Problem for Society1195 Words   |  5 PagesThe problem with teenage pregnancy is it requires intervention from society. This debate is from the perspective of teens, and absent from the medical, ethical, and political views on teen pregnancy. According to Jewell, Tacchi, Donovan, (2000), teen pregnancy is not a problem for teens themselves but more of a problem for society. The large numbers of unintended pregnancies are among unmarried teens. A number of teens do not want to become pregnant, although some are not opposed and other hasRead MoreHuman Nature And Ethics : Abortion Essay1739 Words   |  7 Pages Kylie Morel Ethical Paper- Abortion Due: 11-16-16 Human Nature and Ethics Abortion Abortion, defined by Merriam Webster’s dictionary as, â€Å"the termination of a pregnancy after, accompanied by, resulting in, or closely followed by the death of the embryo or fetus† is a highly discussed and debated topic. The subject has been particularly prevalent in the news recently due to the election of our 45th president and the debates leading up to it. The topic of full term abortions has been a particularlyRead MoreThe Issue Of Teen Pregnancy1722 Words   |  7 Pagescounterpart. But although she may have broken many barriers and achieved great accomplishments, there are still some deep rooted issues which continue to affect her in a most insidious manner. One of the issues that women face in the United States is teen pregnancy which is still an enormous problem that needs to be addressed. The rates are still higher than they were only a decade ago. Becoming a parent permanently and profoundly alters a teenager s life. Most of the girls forget about their dreams ofRead MoreTypes Of Integrative Review784 Words   |  4 Pagesgaps; (e) trend identifiers; and (f) current practices. The search strategy was inclusive of a grey literature as well as a literature search related to specific databases, keywords, and years. The two databases used were Google Scholar and CINAHL; the keywords searched were â€Å"teen pregnancy† or â€Å"sex education† or â€Å"STI Prevention† and â€Å"digital† or â€Å"theory†; and, the years searched were inclusive of five years due to the limited research articles found otherwise. Using the guided questions as a foundationRead MoreSex Education And Comprehensive Sexual Education1609 Words   |  7 Pagesheavily on the topic of sex education, the idea of a pregnant and STD ridden 15 year old tends to frighten a million times more than the thought of proper contraception use and safe sexual encounters. Although the likeliness of a parent wanting their teen to engage in s ex remains extremely low, most would prefer the latter. Comprehensive sexual education should be implemented in all schools because of the misconstrual of information on abstinence-only education, the dire need for parent involvementRead MoreSeveral Cases of Violence Against Children1030 Words   |  5 Pagespoverty, and physical or mental illness. In accordance with social learning theory, I believe that many young teenage girls have these babies because it has become a trend. I remember at one time being pregnant and an unwed mother was looked upon as shameful girls. I knew that becoming pregnant I was no longer able to attend school. In the 1950s and 1960s, the stigma of illegitimacy was such that many young women in their late teens and early 20s were more or less forced to give up their babies. They were

Essay In Cold Blood as an Example of a New Litera Essay Example For Students

Essay: In Cold Blood as an Example of a New Litera Essay ry Form Cold Blood Essays In Cold Blood as an Example of a New Literary Form In an article written in 1966 for The New York Times, Eliot Fremont-Smith discusses the squabbles that occurred in the literary world over Truman Capotes In Cold Blood, squabbles that continue today. He wrote of Capote, The author is now concerned that In Cold Blood be taken as an example of a new literary form, the non-fiction novel'(8). The debate of what constitutes a novel and what constitutes non-fiction. Fremont-Smith argues that the mixing of the two genres is irrelevant: It is too bad, because this fine work raises questions and offers insights that are far more important and, God knows, more interesting than technical debates over the definition of a new or possibly not new literary form. (Book, 8). Fremont-Smith discusses the dichotomy between the moral judgment of an act and the moral judgment of the person who commits it(10). He contends that thinking about this is both frightening and difficult to retain in mind, but that people must keep the act in their minds if they want to come to terms with viciousness of the crime, sorrow for the victims, and sympathy for the victims (10). Others issues are raised as well. Fremont-Smith discusses some of the philosophical questions In Cold Blood raises: Among the matter the book raises-or rather on great sympathy and controlled agony reveals-or haunts the chanciness of our individual existences, how people succumb to or override mutual suspicion, the mystery of how criminals are made and perhaps born, irrelevancy of the legal concept of sanity, the issues of capital punishment and a host of theological questions, as compelling as they are unanswerable. (9) Discussing the merits of fiction versus non-fiction and calling the genre non-fiction novels or even literary journalism is a non-issue. The issue is whether this type of literature is worth while. The Norton Anthology of Postmodern American Fiction contends In Cold Blood blurred the boundary between standard journalism and fiction and could itself create a new layer of narrative tension within the bounds of the traditional novel(Geyh, 125). It has created a new, worthwhile genre with such a narrative tension. Although one can argue that In Cold Blood was not the first book of its kind, it did change literature. Literary journalism is credited to Capote. Many noteworthy books since In Cold Blood have been written in the style of the non-fiction novel. Sleepers by Lorenzo Caracterra, The Last Brother by Joe McGinniss, and Midnight in the Garden of Good and Evil by John Berendt come to mind, but there are many more. The philosophical questions Fremont-Smith contends are raised by Capote are still relevant today. People in Holcomb, Kansas (the setting of the murders in this book) believed they were safe. In their insular world, they believed violence was something that only happened on the football field. Like Columbine High School, safety was only an illusion. Fremont-Smith asked the questions that make us uneasy, the questions that, at the very least, need to be asked, even if we never find an answer. Works Cited Fremont-Smith, Eliot. Books of The Times: In Cold Blood. New York Times Book Review. 10 January 1966. Lexis-Nexis Geyh, Paula, Fred G. Leebron, and Andrew Levy. Postmodern American Fiction; A Norton Anthology. New York: W.W. Norton ; Company, 1998. .

Managing Under Uncertainty Personal Reflection

Question: Discuss about theManaging Under Uncertaintyfor Personal Reflection. Answer: Introduction The group work is one of the most important organizational dynamics in which we can benefit from the cooperative learning. However, it should be implemented properly, without which there would be negative consequences. I was quite afraid at the beginning since there are different individuals with different attitudes in the group. I may not understand the psychological characteristics of the members of the group. There are three members in the group who belong to different countries as well as display different personality attributes. One of them was an extrovert and two of them are introverts. I am an introvert who is unable to interact with others easily. I was apprehensive about the fact that how would the introverts works with a through extrovert. However, when I read the decision-making theories, I was confident that it is possible to arrive at a common decision. There can be a variety of decisions that are taken on a day to day basis. There can be variety of decisions such as decisions regarding uncertainty, complexity, high risk consequences and there can be interpersonal issues (Jaeger et al., 2013). There are programmed decisions as well as non-programmed decisions. The organizations can also engage in routine as well as strategic decisions in every day functioning. There are several tactical decisions as well as operational decisions that need to be taken by the managers (Jaeger et al., 2013). We performed a combination of these types of decisions and had a good experience on the decision-making attributes. The Personality Theory takes into account the inherent characteristics of the individuals that are consistent with their behaviors within a period of time. The Big 5 model states that there are five personality factors responsible for determining the type of individuals. In our group I observed that the introvert members showed signs of conscientiousness as they were organized as well as dependable in their decision making process. They also displayed characteristics of Openness to experience as they appreciated the unusual ideas as well as variety of experiences. The Myers Brigs Type Indicator displays the psychological preference of the people and the way they perceive the whole world. The introverts were systematizer and strategizer. They were quiet, serious, orderly and practical in their decision making approach. They were sensible as well as patient. They were reserved, curious and were action oriented. One of the introverts was judge who used to judge the situations and arrive at a decision. The extrovert in our group displayed the characteristics of performer, socializer and innovator. The performer was easygoing, friendly and possessed practical ability. He was popular, talkative and was attentive to detail. The extrovert was outspoken, quick and often neglected the routine tasks. He was enthusiastic as well as inspiring when presented with difficult situations. The bounded rationality model states that it is possible for efficient decision making by utilizing certain stages (Jaeger et al., 2013). The decision maker finds the instances which needs adequate decision. The next step involves the formulation of possible courses of action. The group members engaged in brainstorming and decide the required courses of action (Jaeger et al., 2013). The extrovert person gave most of the ideas and leaded the group. He engaged the other members to share their own ideas. I was afraid at the beginning and thought that my ideas may face criticism. However, the other members of the group encouraged me to communicate the ideas freely. The group members, after discussion, engaged in selecting the best possible course of action. I was hesitant at first; however, I also gave my interpretation to the group. During the initial period of working in a team, I faced several issues. There was an absence of team identity and we lacked focus while working together. It was difficult for us to get started. We exchanged names with each other and developed an agenda. In spite of these measures, we were unable to start off with our work. One of my group members (an introvert) was not contributing fully to the decision-making activity. He was quiet and was unable to communicate with the other members of the team. There was a conflict that arises in the group regarding the decision to be taken. The conflict was not solved for a long time, which decreased our work performance. There was a cold war which was happening in the team in which the members were unable to communicate freely with each other. This made us unable to focus on our work as we were getting distracted by the conflicts that were taking place in the team. At times, we faced the issue of ineffective leadership and that deviated us from fulfilling our goal. There was poor communication between the team members as the introverts were unable to interact with the other team members. I have learnt that it is important to perform collaborative decision making when acting in a group. The collaborative decision making is a kind of decision making that takes into account the individual opinions while arriving at a decision in a group (Harstad Selten, 2013). At first, I thought that the extrovert person would be dominating when arriving at a decision. However, all the members equally contributed to the decision-making activities. When there was a need for making a decision, we gave individual view points and each one of us gave alternatives. I assumed that the extrovert would not listen to us, however, I was wrong. He gave us the chance to speak and hence there was efficient decision making. I learnt that decision making is relevant when done with several other people. I have learnt that there are many advantages of working in a team. The team work fosters the creativity of the members and it emphasizes on creating innovative products (Schultz Gtz, 2016). It is possible to blend the relative strengths of the employees when working in a group. The working in a group fosters trust among the group members. There is an improvement in the interpersonal relationship between the team members. The team work builds the skills required for effective conflict resolution (Schultz Gtz, 2016). A wide sense of ownership is being created and there is an increase in the workplace effectiveness. I often wondered that individual decision making is a good option and doubted if group decision making is really effective or not. The individual decision making comprises of the thought process of self and its contribution to the decision making process (Sin-Echaluce et al., 2015). I have a tendency to think and I like to question before I perform a particular task. They have certain pros and cons of individual decision making. It saves a considerable amount of time as the individuals engage in fast decision making. My individual decisions are rational as well as focused towards the goals. The individuals are accountable for their own acts which prevent the person from taking wrong decisions. There are several disadvantages of the individual decision making as well. This kind of decision making does not take into account multiple perspectives as there is only one entity involved in decision making. I would use my own views and intuition and hence there is a possibility of inadequate decision making. It does not take into account all the interest of the members and hence the decision may not be appropriate. There are several advantages as well as disadvantages of group decision making activities. We realized the advantages after we successfully completed the team building exercise. There is diversity of opinions in a group which leads to effective decision making. The exchange of ideas as well as opinions enriches the quality of decision making (Kaner, 2014). There is more participation as well as interest of the individuals. The members of the group display positive attributes as it improves the quality of the decision (Kaner, 2014). The collective contribution of the team members makes it possible to remove the factors of unreliability. Our group displayed active participation from all the team members as there was a healthy relationship between the team members. There was democratic decision making in the group which improved the decision-making quality. Our decision was more acceptable as well as perceivable and was well coordinated to meet our objectives. The group discussion helps to develop a large number of innovative ideas which is not possible if there is individual decision making (Kaner, 2014). There are different opinions in a group which expanded the scope of the decisions. Another issue faced by us was that the introvert members were silent at times and they didnt participate in the decision-making process. They were unsure about their opinions and perceive that the opposite person may contradict his ideas. There is also the instance of bias in the decision-making activity. It is possible that the members belong to the same entity which would prevent them from making impartial decisions. There are several techniques that helped us to make decisions in a group. The first method used is brainstorming, which comprises of finding solutions for specific problems by the collection of a list of ideas (Litchfield, 2013). Each one of us gave several ideas aimed for solving the issue in question. We also used a more structured approach such as nominal group technique. The nominal group technique involves the solution generation, identification of problem and the decision making (Harvey Holmes, 2012). We generated the ideas independently and wrote them down in a piece of paper. We didnt interact among ourselves and hence we could avoid the dominance of the extrovert personality. The ideas were collected by our group coordinator and then shared to everyone one by one. We also welcomed any necessary suggestions from the team members. We also adopted a third method of decision making known as didactic interaction, in certain situations. This approach works best when there is a re quirement of only yes-no solution (Liddicoat Tudini, 2013). I found this method of decision making is quite effective in the controlled situation. We engaged in exhaustive investigation as we understood that the selected alternative can have strong consequences. We had also developed a contingency plan that would effectively address the issues that may come up with the decision-making process. I was afraid regarding the way the decision-making activity would turn up. However, I enjoyed during the group work activity as it gave me the opportunity to communicate with few people. I was able to deliver the appropriate decisions that were suitable for the situation. I followed all the essential steps of decision making and implemented them accordingly. We learnt from the mistakes done by us in the initial phase of the decision-making process. This personal reflection would broaden the understanding of the process of decision making. References Harstad, R. M., Selten, R. (2013). Bounded-rationality models: tasks to become intellectually competitive.Journal of Economic Literature,51(2), 496-511. Harvey, N., Holmes, C. A. (2012). Nominal group technique: an effective method for obtaining group consensus.International journal of nursing practice,18(2), 188-194. Jaeger, C. C., Webler, T., Rosa, E. A., Renn, O. (2013).Risk, uncertainty and rational action. Routledge. Kaner, S. (2014).Facilitator's guide to participatory decision-making. John Wiley Sons. Liddicoat, A., Tudini, V. (2013).Expert-novice orientations: Native-speaker power and the didactic voice in online intercultural interaction. Litchfield, R. C. (2013). Brainstorming.Encyclopedia of management theory, 86-88. Schultz, J. S., Gtz, K. (2016). 4 Collaborative decision-making.Ethics and Professionalism in Healthcare: Transition and Challenges, 39. Sin-Echaluce, M. L., Blanco, . F., Garca-Pealvo, F. J., Conde, M. . (2015, August). A knowledge management system to classify social educational resources within a subject using teamwork techniques. InInternational Conference on Learning and Collaboration Technologies(pp. 510-519). Springer International Publishing.

Animal Abuse Problem

Animal Abuse Problem Animal abuse is defined as a deliberate infliction of psychological pain, distress, deprivation, or death of an animal by human beings. Animal abuses occur every time human beings fail to meet the animals’ basic needs. In general, animal abuses comprise of all intentional, socially, and unacceptable behaviors that threatens the welfare of our fellow animals.Advertising We will write a custom essay sample on Animal Abuse Problem specifically for you for only $16.05 $11/page Learn More Such behaviors include kicking, throwing, malicious killing, use of animals for research and entertainment, and neglect and failure to provide an animal with adequate food and veterinary care. Notably, in our factory farms millions of animals such as dairy cows, sheep, pigs, and chickens suffer extremely in the production of food, clothing, and other animal products for human consumption. Surprisingly, many individuals in our society are unaware of these sufferings inflic ted on animals. In the USA, 70% of all human foods comprises of animal products. These animal products are produced behind closed barn doors that house the animals producing them. Before the 20th century, cattle, pigs, and poultry in the USA used to roam in green pastures. However, the increase in human population has forced farm factories to confine these animals to specific cages to meet the ever- increasing demand for animal products. Most meat products are produced in farm industries that confine, mistreat, and deny the animals their natural behavior needs. Although these practices should be unlawful, amendments to the Animal Cruelty Act have seen animals excluded from being treated fairly. As such, these farm factories are almost free to subject their animals to severe suffering with the intention of increasing their products. Dairy cows In developed countries, industrialization of agriculture has seen dairy cows being exploited to meet the increase in demand for milk (Schmid 1 ). As compared to the dairy cows reared 60 years ago, modern dairy cows have been demeaned from cattle to objects. Currently, dairy cows produce 3 times the milk they could have produced at that time. This has been made possible through modern technologies that have focused on improving the animals’ udder size and production. Currently, dairy cows are confined in barns or drylot feed yards. In these cages, the animals are confined indoors for the whole year denying them their right to roam and feel the heat of the sun (Rimas Evan 12). More often, lactating cows are restrained in stalls forcing them to stand in between manure and a mixture of storm water. Dairy farms have adopted genetic selection and selective feeding programs with the aim of increasing milk production. It should be noted that cows’ bodies do not adapt quickly to the high-grained feeds fed to them in the farm factories. Therefore, these feeding methods should be blamed for the increase in metabolic di sorders among the dairy cows.Advertising Looking for essay on environmental studies? Let's see if we can help you! Get your first paper with 15% OFF Learn More Because of being fed and raised in an unnatural environment, these animals have become more vulnerable to the cattle disease that they would have resisted unrestrained. Among the diseases that these cattle have become vulnerable to is mastitis. According to the veterinary experts, millions of dairy cows suffer from the disease causing them to endure painful infections of their udders. After birth, calves are separated from their mothers. Thereafter, male calves are castrated, or killed for veal (Burros 1). Given the fact that these calves are not given opportunities to live their normal lives, human beings should feel compassion for such animals. On the other hand, female calves are raised in special stalls until they are mature enough to be impregnated. In their stalls, these animals are subjected to intense suffering through removal of extra teats, tail docking, and dehorning. In the US, it is estimated that thousands of dairy cow’s calves succumb to scours and diarrhea. Chickens Just like the dairy cows, chickens are subjected to intense suffering in the poultry farms. By being subjected through battery farming methods, chickens are forced to live in tiny cages that do not allow them to turn around. In these cages, the birds cannot stretch their wings, walk around, or sit comfortably while laying eggs (Ginzburg 1). To avoid birds from fighting one another, their beaks are cut off using sharp metals through painful enduring processes. Some animal activists have real footages of poultry workers mistreating these animals. In these footages, some workers have been captured handling the birds in hostile ways, shoving the birds into their cages, and crushing the birds’ feet in the cage doors. Through these footages, it is heartbreaking to imagine the kind of suffering chickens undergo through in their cages. According to some articles, when a bird limb is stuck in the cage parts, the bird is immobilized and if the animal is not rescued in time, it will die a slow painful death. With these kinds of heartbreaking deaths that chicken endures in their poultry farmers, it is high time that consumers should be concerned with the welfare of these animals and campaigned against their inhumane treatments. In the USA, 97% of all the eggs consumed are from chickens reared in cages. The above percentage is disturbing considering the extent of suffering endured by birds reared in battery cages.Advertising We will write a custom essay sample on Animal Abuse Problem specifically for you for only $16.05 $11/page Learn More Pigs In farm industries, pigs are the other victims of animal suffering. Immediately they are born, they are forced to bear agonizing surgical procedures such as tail docking and castration (Bdale 1). Afterwards, they are denied their chance to be with their mother by being taken away and locked in crowded enclosures. While pregnant, their mothers are locked up in tiny cages for up to 16 weeks. Usually, the cages are so small that the animals cannot walk around or turn around. In the cages, these animals have to endure boredom and depression. Prior to giving birth, the animals are relocated to smaller cages referred to as farrowing crates (Bdale 1). When they give birth, the piglets can suckle from outside the farrowing crates. Since their mother is trapped between the tiny cages, the piglets can hurt her nipples without her defending herself. Based on the above real experiences, it is heartbreaking to imagine the extent of pain pigs have to endure through to meet the pork demand. Human beings should realize that pigs feel pain, undergo through stress, and suffer just as our pets. Therefore, we should look for better ways of reducing and ending the suffering, we have subjected pigs in our factor y farms. An example of an organization aimed at reducing animal suffering In the USA, several organizations have been formed to attempt to reduce the suffering of animals in farm industries. American Humane is such an organization formed with the aim of safeguarding children and animals’ welfares. This nonprofit organization has been concerned with the way animals are being treated in our farms. Currently, through its extended programs the organization is engaged in trying to improve farm programs. Through this program, the organization aims at ensuring that farms certified under their programs meet the required animal welfare standards. Despite the relentless efforts of such organizations to end animal suffering, billions of animals continue to die and suffer from the inhumane conditions of our factory farms. According to underground investigations, most animal products for human consumption are the products of horrific deaths.Advertising Looking for essay on environmental studies? Let's see if we can help you! Get your first paper with 15% OFF Learn More It is facts that as long as human beings are going to depend on animal products for consumption animal suffering will continue to persist. In this regard, animal activists, governments, and the consumers should be committed to preventing, ending, and denouncing animal suffering. Equally, appropriate regulations should be formulated to lessen the suffering and improve the welfare of these animals. Researchers should work tirelessly to ensure they design appropriate facilities for farm animals. These facilities should be stress free and enhance the welfare of the animals. Similarly, appropriate slaughtering methods should be designed to reduce the pain endured by animals in slaughterhouses. In conclusion, human beings should note that they are morally obligated to let animals enjoy the brief lives before they are slaughtered. As such, it will be more appropriate if animal farms allowed these animals to roam freely in their habitats. Equally, animal slaughterhouses should adopt ethical means of slaughtering these animals. Likewise, if we humans preach and practice civilized morals in our society, animal suffering in our farm factories should be put to an end because animals have every right to enjoy their existence as we humans. Burros, Marian. Veal to Love, Without the Guilt NYTimes.com. The New York Times Breaking News, World News Multimedia . Version 1. N.p., 18 Apr. 2007. Web. https://www.nytimes.com/2007/04/18/dining/18veal.html?searchResultPosition=1mtrref=www.nytimes.com. Bdale, David. Factory Farms Revealed: Animal Abuse and Cruelty | The Last Newspaper. The Last Newspaper | Rowan University Comp 1 Fall 2010 Hodges. Version 1 . N.p., 13 Oct. 2010. Web. Ginzburg, Ralph. ANIMAL-RIGHTS GROUP ASSAILS EGG FARM New York Times. The New York Times Breaking News, World News Multimedia. Version 1. N.p., 17 Jan. 1988. Web. https://www.nytimes.com/1988/01/17/nyregion/animal-rights-group-assails-egg-farm.html?searchResultPosition=1. Rimas, Andrew, and Evan D. G . Fraser. Beef: the untold story of how milk, meat, and muscle shaped the world . New York, NY: William Morrow, 2008. Print. Schmid, Ronald F. The untold story of milk: the history, politics and science of natures perfect food : raw milk from pasture-fed cows. Rev. and updated. ed. Washington, DC: NewTrends Pub., 2009. Print.

Prostanoids Essays

Prostanoids Essays Prostanoids Essay Prostanoids Essay Introduction 1. The Prostanoids Prostaglandins, the cyclic lipid go-betweens that belong to the ace household of eicosanoids ( Gryglewski, 2008 ) are 20 C fatty acerb merchandises of the additive polyunsaturated Arachadonic Acid ( AA ) ( Dey et al,2006 ) ( a cis-5,8,11,14-eicosatetraenoic acid ) . Often referred to as local short scope endocrines due to their metabolic and chemical instability ( half lives of Pgs is 30 seconds ) ( Kazuhito et al, 2002 ) the prostaglandins can be found in about every mammalian organic structure tissue where they maintain local homeostasis. ( Tsuboi et al, 2002 ; Coleman et al,1984 ) Certain structural characteristics that are outstanding amongst the prostanoids include carboxylic acid ; a hydroxyl group at place 15 and 2 aliphatic side ironss ( Manabu et al. , 1995 ) ( Gryglewski, 2008 ( Narumiya et al, 1999 ) . Arachidonic acid, enzymatically released from the phospholipid shops by PLA2, figure 1, is metabolised by the PGH synthases, COX 1 and 2 ( Dey et al,2006 ) ( the former referred to as constitutive is found in blood thrombocytes, the latter is known as inducible and is found in inflamed tissues ) to bring forth the prostaglandin endoperoxides PGG2 and PGH2, which depending on the tissue will metabolize farther by either the thromboxane/ prostacyclin synthases to PGD2/PGE2/PG12/TXA2 or reductases ( to synthesis PGF2 i? ) . ( Gryglewski, 2008 ) Due to their synthesis from fatty acids in the cell membrane ( which allowed them to go through through the membrane and range neighboring cells ) the prostanoids were originally thought to be hydrophobic but subsequently surveies subsequently concluded this is nt wholly true and that prostaglandins like PGF2i?iˆ are really H2O soluble. Figure The synthesis of Prostanoids by the use of Arachidonic acid by assorted enzymes including the PGH/PGG synthases. Besides included are some of the signal transduction and physiological actions of the receptors. Prostanoids exert a assortment of actions including neuromodulation ( PGE2 ) and platelet collection ( TXA2 ) ( Negishi et al, 1995 ) ( Coleman et al, 1984 ) that are mediated by their receptors ( situated on the plasma membranes ) which belong to the G protein coupled visual purple type ace household ( Dey et al. , 2006 ) of cell surface receptors. Each receptor has 7 hydrophobic putative transmembrane spheres within which the 3rd/ 7th transmembrane spheres every bit good as the 2nd extracellular cringle are conserved. ( Manabu et al. , 1995 ) . Certain constructions thought to be of import for the binding of prostanoid molecules include Arginine. ( Negishi et al, 1995 ) . The of course happening prostanoid receptors ( briefly described in the undermentioned subdivision ) were originally characterised in bioassay systems on assorted smooth musculus readyings ( table 1 ) and so harmonizing to their sensitivenesss to the major prostaglandins ( table 2 ) ( Negishi et al, 1995 ) . Secondary courier systems ; MRNA splicing discrepancies ( Jones et al, 2009 ) and ligand adhering surveies have besides been used in both receptor word picture and the find of the EP receptors subtypes, EP 1-4 and their isoforms ( EP3 is thought to hold up to 7 isoforms ) . Function of cistrons encoding mouse TP ; EP ( 1-4 ) ; IP ; FP and DP receptors revealed they are on chromosomes 10, 3, 15,7, 3, 8 and 14 severally. ( Narumiya et al, 1999 ) Smooth musculus response to receptor activation Receptors involved Contraction TP EP1 FP EP3 Relaxation IP EP4 EP2 DP Table Word picture of the receptors harmonizing to the type of response elicited on a smooth musculus ( Walch et al, 1999 ) Receptors Prostaglandins which have the greatest activity Displaced person PGD2 EP PGE2 FP PGF2i? Information science PGI2 TP TXA2 and its precursor PGH2 Postpone The original word picture of the receptors harmonizing to their sensitiveness towards the prostaglandins. 1.2 Naturally happening Prostaglandin receptors PGD2, the major prostanoid for DP receptors is formed in many tissues and cells and produces a assortment of biological responses including suppression of thrombocyte collection ( other more powerful DP agonists moving in a similar mode include 9-Deoxy PGD2 ) ; relaxation of vascular and non vascular smooth musculus ; alteration of autonomic and centripetal nervus maps every bit good as the intercellular ordinance the liver cells map ( Manabu et al, 1995 ) . DP receptors mediate their relaxant actions by triping adenylate cyclase and increasing intracellular camp degrees ( Manabu et al, 1995 ) . The major prostanoid for the FP receptors is PGF2a, known as the primary PG. More powerful agonists, Cloprosterol and Fluprostenol, were developed by ICI Pharmaceuticals as possible preventive agents in adult females. PGF2a actions include compressing smooth musculus readyings ( bronchea and windpipe ) and bring forthing a mitogenic response in civilized cells ( e.g. Osteoblast cells ) . FP receptor adversaries may be used in bar of pre-term labor ( Jones et al, 2009 ) . Signal transduction surveies indicated cloned FP receptors couple Gq ensuing in the activation of PLC. PGF2a stimulated phosphoinositide metamorphosis and activation of PKC besides occurred through FP receptors and led to the initiation of programmed cell death in cells. Thrombosis/haemostasis are thought to be mediated by TP receptors ( Manabu et al, 1995 ) which come in 2 isoforms, i? and i?iˆÂ ¬ and were isolated from purified blood thrombocytes and placenta utilizing high affinity specific adversary ( S-145 ) . TXA2, the of course happening TP receptor agonist is involved in cardiovascular disease ( e.g Acute MI ) . For experimental intents more stable agonists such as U-44619 are frequently used. ( Jones et al, 2009 ) TP receptors are thought to match at least 4 G-proteins ( Gq/Gi/G12/G13 ) which in bend leads to the activation of the undermentioned 2nd couriers and effecters: Ca2+ ; IP3/DAG ; camp ; PKC and PKA which later can take to platelet aggregation/ form alteration. The TP adversary BMS 180,291 is normally used in surveies as it is both powerful and orally active in add-on to being long playing and extremely selective. ( Misra et al. , 1992 ) The IP receptor agonist PGI2 maintains homeostasis in the organic structure by opposing the actions of TXA2 ( e.g. platelet collection ) . PGI2 itself is labile hence the more stable Cicaprost is used in surveies ( Manabu et al, 1995 ) . IP receptor mediated actions include loosen uping arterial smooth musculus ; platelet aggregation/degranulation and form alteration suppression ; vasodilatation of the pneumonic beds and modulating nephritic blood flow. IP receptor is thought to match the undermentioned G protein ( ) exert its actions by assorted effecters as shown by the following signalling tract: IP receptor activation leads to stimulation of adenylate cyclase and later an addition in intracellular camp which at high degrees, through PLC activation, causes calcium mobilization or pkA cascase. IP adversaries could potentially be used for hurting direction ( Bailey et al, 1998 ) . Two lead IP adversaries include RO-1138452- portion of the 2- ( phenyl amino ) -imidazoline series- and compound 21 ( Clark et Al, 2004 ) . Functional surveies utilizing guinea hog aorta/human pneumonic arteria revealed pA2 values of 8.39/8.20 for RO-11384520. The equilibrium dissociation invariable: Schild Vs Cheng-Prusoff Isolated tissue checks are critical to the survey of adversaries ( this includes finding of their affinities ) every bit good as the pharmacological distinction of receptors. Some of import footings associating to this kind of survey are KB the equilibrium dissociation invariable ; pA2 the measuring for antagonist activity and must be a positive figure ; EC50 the agonist concentration required to bring forth a bomber maximal ( 50 per centum ) action on a concentration consequence curve in the absence of adversary and IC50 the concentration of adversary needed to cut down the consequence of an agonist by 50 per centum. The chief methods used to mensurate KB are the Schild and Cheng-Prusoff analysis nevertheless other theories include those by Gaddum ; Guarino and Bovet. Schild analysis Pharmacologists originally determined KB by using schild analysis ( Cheng, 2002 ) to concentration consequence curves constructed for an agonist in the presence and absence of several antagonist concentrations ( Arunlakshana and schild, 1959 ; Schild,1947 ) nevertheless a individual adversary concentration can besides find KB ( individual point finding ) ( figures 2 and 3 ) ( Van Rossum, 1963 ) . Schild s most of import finds are finding the affinity of a competitory adversary and recognizing the significance of the dosage ratio ( despite the agonist action being unknown ) . ( Calderone, 1998 ) The dose ratio ( A1/A0 ) is the factor by which agonist concentration A0 in the absence of an adversary must be increased by in order to bring forth the same response as concentration A1 in the presence of an adversary. ( Calderone, 1998 ) Figure: Dose response ( hypertext transfer protocol: //www.curvefit.com/schild.htm ) Figure: Schild secret plan ( hypertext transfer protocol: //www.curvefit.com/schild.htm ) The Schild secret plan was besides originally used to find KB ( kenakin, 1982 ; schild, 1947 ) from individual point finding surveies but was subsequently found to give the correct reply in a wider scope of state of affairss therefore connoting theoretically a typical competitory adversary should bring forth parallel displacements in agonist response concentration curves with no suppression of the soap response every bit good as a negative incline of integrity when log ( concentration ratio -1 ) is plotted against -log ( antagonist concentration ) . The Schild method is one of the nothing methods which are used in functional surveies and there are many advantages to this ; for one there are restrictions in functional surveies such as unknown agonist tenancy but the void method overcomes this as it considers that a certain response is elicited from a certain sum of agonist tenancy in malice of the antagonist tenancy. Looking at the equation, the concentration, affinity and efficaciousness of the agonist are all irrelevant plus the dose ratio does nt trust on the nature of the agonist and magnitude of response. Therefore one considers Schild to be simple yet effectual. Overall Schild is thought to be a pharmacologically valid yet robust ( Colquhon, 1987 ) method even when there are non adequate receptors to make a direct binding experiment or when the receptors are excessively heterogenous ( Kenakin, 1984a ) to enable reading of ligand binding experiments ( although if a specific agonist was available so Schild could be used to an alyze the receptors that elicit the response ) . ( Lazareno and Birdsall, 1993 ) Schild analysis still has flaws nevertheless and these include its inefficiency and demand for tonss of informations points. Cheng- Prusoff and the Inhibition curve Inhibition curves ( figure 3 ) are routinely used in 2nd courier checks and affect increasing concentrations of an adversary to cut down the agonist concentration. ( Bockaert et al,1990 ) . Important parametric quantities for an suppression curve include the location parametric quantity ( I50 ) -originally thought to depend on the agonist concentration used-which can be converted to the affinity changeless Ki ( appendix B ) by the original Cheng-Prusoff equation in a monosubstrate enzyme reaction. ( Cheng, 2002 ) Figure: Example of an Inhibition curve on which the Cheng-Prusoff analysis can be applied. The location parametric quantity ( IC50 ) can be determined and so converted to Ki. ( hypertext transfer protocol: //journals.sfu.ca/coactionbks/index.php/Bindslev/article/viewArticle/6/67 ) Cheng-Prusoff protocol ( see Appendices B and C for derivations ) applies chiefly to enzymatic reactions nevertheless few prostanoid receptor surveies have used it including Bley et Al ( 2006 ) who calculated pA2 values for IP antagonists RO-1138452 and RO-3244794. There are premises made from the equation such as both substances interact at a individual binding site harmonizing to the jurisprudence of mass action and besides the substance s free concentrations are known and changeless throughout the experiment. ( Lazareno and Birdsall, 1993 ) . The Cheng-Prusoff analysis is frequently criticised ( see Munson and Rodbard, 1988 ) yet in pattern is used alternatively of Schild as it allows a lower scope of concentrations to be used and the antagonist action can be seen clearly even when the steady province is easy reached for case by adversaries that have a high affinity ( Jones et al.,2009 ) or are extremely lipotropic ( Jones et al.,2008 ) . This is in add-on to the fact that is can be used for dearly-won or limited agonists. Furthermore the easiness of building an suppression curve makes it more appealing. The job with the analysis is the inappropriate interlingual rendition of the original equation from the linguistic communication of enzymology to pharmacological medicine and the uncertainness of whether the agonist affinity invariable is needed to find the adversary s affinity invariable, Ka ( something Craig,1993 found unneeded as KB was in existent fact independent of it ) . Radioligand adhering surveies ( see Appendix A ) are complicated by the fact that EC50 does non equal Ka. unless a additive receptor tenancy response relationship exists ( Craig, 1993 ) hence te look is altered and ( KB =I50/1+ ( A/ Ka ) is used to change over Ka to Ec50 ( when the interaction with the receptor follows bimolecular dynamicss ( Craig,1993 ) . Further jobs with this technique include the trouble in implementing the equation in fortunes where small information exists about the agonist ( for case its concentration ) . Leff and Dougall ( 1993 ) stated there were no set standards for Cheng- Prusoff to prove an adversary of true competitory nature unlike Schild, this is in add-on to the trouble in separating between a competitory and non competitory adversary if the suppression curve was used ( something evidently detected with a Schild secret plan ) . Eglen and Whiting ( 1989 ) suggested using Cheng- Prusoff at a high agonist concentration is invalid which contradicts Lazareno and Roberts ( 1987 ) theory. erroneous KB values ( Leff and Dougall, 1993 ) . It is critical hence to cognize how to construe the cheng prusoff equation in a pharmacological scene and to understand the importance of KB and I50 and their dependance on the form of the curve. ( see Appendix ) With their complex pharmacological medicine, possible curative value and appropriate mathematical agencies of characterizing receptor ( every bit good as finding agonist/antagonists authorities ) there has been a batch of involvement in the prostanoids. EP receptors The EP receptor system is of involvement in this survey, with 4 subtypes ( and a figure of isoforms ) identified and known to intercede actions of an opponent nature ( e.g. stimulation and suppression of neurotransmitter release or inhibition/stimulation of Na and H2O resorption in kidneys ) through assorted complex signalling transduction mechanisms, Negishi et Al ( 1995 ) some of which have still to be clearly identified, it is of no surprise that the EP receptor system is the Centre of many surveies including this 1. The EP receptor was pharmacologically divided into four subtypes ( EP1/ EP2/EP3 and EP4 ) based on their responses to assorted agonists and adversaries. Each subtype can match a member of the g-protein coupled receptor household in order to excite a signal cascade. EP1is best characterised by its limited tissue distribution and repressive actions on intestine and trachea smooth musculus. Northern Blot analysis revealed both EP1/3 receptors were expressed in the kidneys ; lungs and uterine. Protagonists known to adhere EP1receptors have been ranked in the undermentioned order harmonizing to their authorities: 17-phenyl-PGE2 A ; gt ; PGE2 A ; gt ; sulprostone A ; gt ; iloprost ( partial EP1 agonist ) . EP1 receptor adversaries include SC-51322 which has a pA2 of 8.8 and schild secret plan incline of 0.91 ( bespeaking competitory hostility ( Durocher et al, 2000 ) and ONO-8711 ( which besides shows some EP3 hostility ) . EP2/4 receptors are abundant in ileum, Thymus, kidneys and bosom severally and intercede vasodilatory actions on blood vass every bit good as loosen up the windpipe and ileum. ( Negishi et al,1995 ) Protagonists used to trip these receptors include Butaprost ( selective EP2 agonist which is insensitive to EP4 ) and PGE2 ( Negishi et al, 1995 ) . Adversaries for EP2/4 receptors are as follows: AH6809 ( EP2 adversary and its a weak ligand for murine EP1 and DP1 ) and AH-23848-the lead EP4 adversary which can distinguish between EP2/4 and IP agonism ( Jones and Chan, 2001 ) and CJ-023,423/L-161982 ( EP4 antagonists with a pA2 of 8.3 the latter portions some structural characteristics with ep3 adversaries ) . In footings of signal transduction it is thought that EP1 couples an unknown G protein which leads to PKC activation which later, when over expressed, leads to desensitisation of EP1 receptors. During its phosphorylation ( possibly in 3rd intracellular cringle ) in worlds nevertheless Calcium mobilization is inhibited. EP4 positively couples Gs taking to its relaxant actions and both EP 2/4 receptors stimulate Adenylate cyclase and later addition degrees of Ca managing taking to EP2 repressive actions. EP4 mediated PGE2 actions ( e.g. suppression of function/proliferation of T cells ) are via an intracellular camp. Unlike the other receptors EP3 has been extensively studied and is the subtype receptor we shall concentrate on for this undertaking. A widely distributed inhibitory receptor ( found in kidneys, encephalon, GI piece of land and womb ) it exerts assorted pharmacological actions including the contraction of smooth musculus ( vascular and uterine ) , suppression of lipolysis and sweetening of thrombocyte collection. These belongingss are of import as there is a strong deduction that ep3 receptors and their isoforms are involved in cardiovascular diseases ( e.g. MI/ tachycardia/ high blood pressure ) . EP3 has a complex pharmacological medicine as it mediates its actions through assorted effecters and secondary couriers including Adenylate cyclase ( its suppression takes topographic point when phosphoinositide metamorphosis is stimulated ) ; camp ; the initiation of Ca inflow ( which later leads to chloride channel suppression ) ; PLC activation and Ca mobilization. Noradrenaline/ serotonin release is besides thought to be affected by the ep3 receptor. The G-proteins that couple EP3 receptors are Gi and Gq. Protagonists most normally used in ep3 receptor surveies are TEI-3356 ( a selective EP3 agonist ) and sulprostone ; ONO-AE-248 ; PGE2 and 17-phenylPGE2. Compounds used to barricade EP3 agonists include lead compound DG-041 ( an AT1 adversary that has already been investigated in a stage 1 clinical test ( Heptinstall et al, 2008 ) which is thought to be a utile for future intervention of peripheral cardiovascular disease by suppression of adenylyl cyclase/priming of protein kinase C ( Matthews and Jones, 1993 ) and L-798106 which blocked pre synaptic EP3 inhibitory sulprostone actions in guinea hog vessel deferens ( pA2 values for this compound are 7.5 and 7.8 severally ) ( Jones et al.,2009 ) . In add-on to this it was found to hold caused a parallel supplanting of log concentration response curves for sulprostone mediated rat femoral arteria contraction ( Hung et al, 2006 ) . Schlemper et Al ( 2005 ) implied L-826266 had some indirect repressive actions on EP3 but this has nt been to the full demonstrated. Both L-826266 and L-798106 did nevertheless barricade EP3 contraction in guinea hog aorta but it s been suggested that the groun ds behind the oncoming of the obstruction being so slow are related to the high lipophilicity of the compounds as opposed to their affinity. ONO-AE3-240, another EP3 adversary was extremely EP3 selective compared to EP1. ( Amano et al, 2003 ) The job with EP3 and prostanoid adversaries in general has been in their word picture which is both hard and dearly-won. As possible medical specialties there are a batch of restrictions, for case belley et Al ( 2006 ) showed their adversaries to be extremely protein edge which accordingly decreased their activity SAR surveies have suggested that indole karyon incorporating compounds retain a high affinity for EP3 after inversion of the indole karyon. Indolones and hexahydro-indolones ( OConnell et al, 2009 ) were besides said to hold high EP3 affinity. In add-on to this saturating alpha-beta bond allows for a high authority of EP3 adversaries nevertheless farther impregnation of dual bonds in hexahydro-indolone residues consequences in reduced affinity. Despite the fact that there are chemicals available, research is still on traveling to happen for case a more selective agonist and adversary such as 3,7-dithiaPGE1, the lead ep4 receptor agonist that was discovered through structural alteration. 3,7-dithiaPGE1 is an acidic molecule derived from the PGE1 molecule. Using the basic PGE1 skeleton the heteroatom Sulphur was added to the 7 C place which led to potent affinity for assorted receptors ( ep4, ep3 and information science ) . Another sulfur was so introduced into the a concatenation at the 3 C place which resulted non merely in good ep4 receptor selectivity and agonist activity but besides powerful ep3 receptor activity with a qi of 220nM and ec50 of 1.1nM. The debut of a 2nd sulfur besides led to decrease affinity for the IP receptor. Although there is some ep4 activity the chief issue with 3, 7-dithiaPGE1 prevarications in ep3 receptor affinity and activity as the writers have stated contrasting values for the qi and EC50. The purpose therefore of this survey is to find the selectivity in relation to its ep3 activity. As there are few literature that have studied this chemical, the usage of tissues that are known to hold a great trade of ep3 receptors and can react good to them are needed, hence, usage will be made of the guinea hog vessel deferens and aorta. For vascular surveies the human pneumonic arterias are appropriate nevertheless they are prone to tachyphylaxis and so small information can be gained from them during check surveies therefore the Guinea Pig Aorta and Vas deferens are used routinely. Although there is small correlativity between the tissues in footings of agonist sensitiveness there is a strong nexus between the contractile authorities of EP3 on Aorta/vas and pneumonic arterias. In add-on to this the Guinea Pig is comparatively cheap compared to other animate beings. Furthermore Vas Deferens ( a good studied tissue in footings of nervus transmittal ) is thought to be more sensitive to EP3 agonists than aorta. In add-on to this it s thought that PGE2 synthesis and distinction of receptors took topographic point on Vas Deferens ( Trachea and Ileum are besides accountable for the latter ) ( Coleman et al, 1984 ; Jones et Al, 1986 ) . To research the EP3 effects of 3,7-dithiaPGE1 in isolation requires the usage of other agonists and adversaries and this is due to the fact that there is an copiousness of other receptors in both the aorta and vessel deferens for illustration TP/EP1/i?1 which are to be characterised in the tissues therefore the undermentioned agonists and adversaries will besides be used: 17-phenylPGE2 ; BMS-180291 ; Phenylephrine ; PGE2 and ASA-3ap. 2. Methods and Materials Isolated tissue readyings Forfeit of the lab animate beings was performed harmonizing to the UK Home office agenda 1 guidelines. Male Dunkin Hartley guinea hogs of 300-350 gms were euthanized by inhaling 100 % CO2. The tissues were so instantly dissected out. The falling thoracic aorta was removed and cut into rings, which were measured at about 3mm in length, and were stored in oxygenated Krebs buffer solution ( room temperature, gassed with 95 % CO2 and 5 % O2 at a changeless rate to keep a pH of 7.4 ) which was made of ( millimeter ) NaCl 118.4 ; KCL 4.7 ; NaHCO3 25 ; Glucose 11.1 ; MgSO4 1.2 ; NaH2PO4 1.2 ; CaCl2 2.5. The tissue was so transferred into a 20 milliliter bath and supported by two chromium steel steel wires in order to mensurate tissue tenseness. Isolating the Vas Deferens required the venters to be cut unfastened and the tissue removed from the testicle. Any extra fat, connective tissue, blood vass and mesentery were so removed suitably. The tissue was once more immersed in warm Krebs solution. On a gel home base the tissue was pinned down suitably in order to bind a knot at each terminal, a cringle was so made at one terminal in order to suspend the tissue ( in a conventional 10ml organ bath ) between the transducer and wire electrode. The readyings were ace fused at a changeless rate with oxygenated Krebs solution set at temperature of 37oc by the usage of gravitation provender setup. Prior to suspending the tissues the isometric transducer was calibrated and the resting tenseness applied to both vessel deferens and aorta was 1g. After the setup was set up with the tissues the necessary alterations to weave tenseness were measured in gms by the isomeric transducer ( Dynamometer UF1, Pioden Controls ) connected to a CED 1902 MK III amplifier ( CED Science Park, Cambridge, England ) . The Intracept- Chart V.4.8.1 bundle on a Dell desktop computing machine was used to enter the informations. Protocols Aorta. For every experiment once the tissue was set up on the setup a force of 1g resting tenseness was applied. Furthermore anterior to adding any other chemicals 100 nm BMS 180291 was added to the bath and allowed to incubate for 10 proceedingss in order to suppress TP receptor activity. The COX-2 inhibitor Indomethacin ( 0.5 milliliters of 20i? ­M used ) was besides added to the Krebs reservoir ( 10 L which was split between the pupils in the group ) in order to barricade any endogenous TP activity. A cumulative dosage response curve was foremost constructed by adding 100, 300, 1300 and 3300nm phenylephrine ( four- five proceedingss were left between each dosage in order to let for a full consequence to be seen ) so the dosage that gave a close maximal contractile response ( about 60-70 % ) of the tissue could be used for priming anterior to adding the prostanoid agonists and adversaries of involvement. The readying was so washed 3 times before go oning with the following measure. Once primed with 100nm of BMS 180291 and Phenylephrine ( usually 900nm -1i? ­M was added ) for about 15 proceedingss a cumulative dosage response curve was constructed utilizing 17- phenylPGE2 ( 1nm, 5nm and 25nm were added at 5 minute intervals ) in order to mensurate the contractile response. This measure was repeated except with the usage of 3, 7-dithiaPGE1 ( 100, 500 and 2500nm ) . ASA-3ap was besides added in the concluding measure. Vas Deferens. At the start of each experiment the resting tenseness was recorded in a similar mode to aorta. Prior to adding any drugs the stimulator was set as follows: group interval ( 40 seconds ) , pulses per group ( 10 ) , pulse frequence ( 10Hz ) , pulse breadth ( 1ms ) , pulse amplitude ( 5v ) and a filter was on if required. A scope of electromotive forces ( 30v-70v ) were applied to the tissue to see if it contracted ( anterior to adding any repressive agents ) and the electromotive force which gave a close maximal contractile response ( most frequently this was at 60v ) was used for future experimentation. Initially a individual electromotive force ( 60v ) applied to the tissue and so a individual dosage of PGE2 ( 5nm ) was added to see if the tallness of contraction decreased ( this indicated that the chemical did in fact inhibit tissue contraction as expected ) . The readying was so washed out ( this had to be done in the 40 2nd interval ) several times before go oning wi th the experiment. The first protocol involved the same electromotive force applied once more and PGE2 added in a cumulative mode ( 3, 10 and 30 nanometer ) to suppress the contractions. The protocol was repeated once more but with 3, 7-dithiaPGE1 ( 100, 300, 500 and 700nm ) added in a cumulative mode. The 2nd protocol involved adding ASA-3ap ( 100nm ) which was allowed to incubate for 10 proceedingss so PGE2 /3, 7-dithiaPGE1 were added in the hope to suppress the action of the adversary. Datas Analysis Data was collected and recorded via the Intracept chart V4.8.1 bundle. For each new experiment the tissue tenseness was measured before and after adding the tissue ( in gm ) . To cut down biological fluctuation between stray tissue readyings the consequences ( e.g. contractions inhibited by 3, 7-dithiaPGE1 ) were normalised by being expressed as per centums of the tissues maximal contractions ( this was determined by mensurating the tallness of the contraction ) in the absence of agonists and adversaries. Graph pad Prism version 4 was used to build log concentration dosage response curves. The curves were fitted utilizing the undermentioned options: variable sigmoidal dose response, restraining the curve to the initial tone at 100 % and if necessary at resting tenseness ( 0 % ) . Data was expressed as the mean iˆÂ «iˆ?iˆÂ ­ the standard mistake mean of n independent probes. Statistical analysis was besides carried out by utilizing Graph tablet Prism, version 4 and Graph pad instat 3. Statistical significance was assumed if P A ; lt ; 0.05. Chemicals used The chemical of involvement in this probe, 3, 7-dithiaPGE1, was supplied from Allergan, USA. Likewise PGE2 and 17-phenyl-w-trinor PGE2 were supplied from Cayman Chemical, USA. ASA-3ap ( 1- ( 3-methoxybenzyl ) -3a-methyl- [ 3, 3a, 4, 5, 6-hexahydroindol-2-one-7-acrylic acid, 3, 4-difluorobenzenesulphonamide, 10 i? ­M in DMSO ) was provided by Target Molecules, UK. BMS-180291 ( [ 1S- ( exo, exo ) ] -2- [ [ 3- [ 4- [ ( pentylamino ) carbonyl ] -2-oxazolyl ] -7-oxabicyclo [ 2.2.1 ] hept-2-yl ] methyl ] -benzenepropanoic acid, Ifetroban ) was provided from Bristol-Myers Squibb, USA. Indomethacin ( 20i? ­M ) in ethyl alcohol was bought from Sigma-Aldrich, USA. Phenylephrine was provided by the University of Strathclyde. The stock solutions were prepared by the undermentioned procedure ( this applied to all chemicals except ASA-3ap ) : Indomethacin was dissolved in ethyl alcohol to accomplish a concentration of 20i? ­M ; the prostanoid agonists and adversaries ( PGE2, 17-phenyl-w-trinor PGE2 and, BMS-180291 ) were besides prepared in a similar mode except to concentrations of 1mM ( BMS 180-291 ) ; 1, 10 and 100 millimeter ( PGE2 ) and 17-phenyl-w-trinor PGE2. Stock solutions were so diluted suitably with 0.9 % NaCl solution ( saline ) . For ASA-3ap the first dilution was solubilised with a hint of NAHCO3. Consequences Guinea Pig Aorta- conventional experiments Phenylephrine cumulative dose response curve. Phenylephrine was added cumulatively to the stray tissue to show the tissue s response to phenylephrine in a concentration dependent mode. Figure 1 Log concentration response curve for phenylephrine in stray guinea hog aorta. The consequences are expressed as a per centum of the maximum tissue response. Establishing the being of an EP3 receptor system in stray guinea hog aorta. Prior to cumulatively adding 3, 7-dithiaPGE1 a priming dosage of phenylephrine was established by gauging the dosage that gave an appropriate EC20 ( the dosage that produces a response that is 20 % of the maximal response ) . This frequently varied between 500nM and 1300 nanometer nevertheless more frequently than non 1000 nanomet

Those who would give up freedoms for temporary security deserve Thesis

Those who would give up freedoms for temporary security deserve neither, civil right violations - Thesis Example After the civil rights movement people in America became more aware of their rights than ever and more willing to battle the government to protect these rights. Despite the advances made the 1960’s America currently faces many threats to the civil rights of its citizens in areas such as freedom of speech, illegal search and seizure and the rights to bear arms. Freedom of speech is sacred privileged given to every US citizens by the forefathers of this nation when they wrote the constitution of the United States of America. The first amendment of the constitution which is part of the bill of rights states â€Å"Congress shall make no law respecting an establishment of religion, or prohibiting the free exercise thereof, or abridging the freedom of speech or the press; or the right of the people to assemble and to petition the government for a redness of grievances† (Usconstitution, 2009). Despite the existence of such a powerful abiding law concerning freedom of speech violations to this constitutional right have occurred many times throughout America’s history. Music is a form of expression that allows artists to express their thought and feelings their loyal fans. Music unites people and provides musicians with the ability to send social messages that can change their environmental surroundings. In the 1990’s a new genre of music was born called Gangster Rap. This new form of rap utilized violent lyrics and sexually explicit material which many segments of our society did not like. In 1990 Missouri introduced a bill that forbids the sale of records containing lyrics that are violent, sexually explicit, or perverse (Nuxum, 2003). Some of the first artists that were affected by this complete violation of the freedom of speech privileges Americans enjoy were Two Live Crew, Dr. Dre, Snoop Dogg, and Ice Cube. Sometimes violations to the freedom of speech privileges occur in the most unlikely